NICED : Scientists

Dr. Santasabuj Das

Dr Santasabuj Das is currently appointed as Scientist E at the National Institute of Cholera and Enteric Diseases (NICED), Kolkata. He is also the principal investigator and coordinator of the Biomedical Informatics Center of ICMR at NICED. He has the academic and professional background in Clinical Medicine and is pursuing a full-time research career since 1998. He joined NICED as an independent investigator and a permanent employee in January 2005. His current research interest spans several areas of host responses to microorganisms (both pathogens and commensals) including immune responses and vaccine development. The major focus of his research is the pathogenesis of human Salmonella infections, development of subunit and conjugate vaccines against Salmonella Typhi and Paratyphi and the regulation of mucosal immune responses at the intestine during homeostasis as well as under disease conditions, such as intestinal infections and inflammatory bowel diseases. Dr Das extensively uses computational tools, in vitro and in vivo techniques and clinical materials to address his research questions. His laboratory has developed a novel mouse model of Salmonella Typhi infection. Using this model, his research has identified several virulence factors critical for the pathogenesis of Salmonella Typhi and developed a candidate subunit vaccine. His research has unearthed a number of novel signalling mechanisms that underlie pro-apoptotic and anti-cancer role of dietary fibres and regulate intestinal innate immune responses by regulating the expression of Toll-like receptors and small cationic antimicrobial peptides. His research has been funded by extramural grants from all the major funding agencies of the country (ICMR, DBT, CSIR, DST, DHR) as well as the Ministry of Education, Culture, Sports, Science and Technology of Japan and Japan Agency for Medical Research and Development. Dr Das has published 43 original research articles and 3 review articles in reputed international journals, 2 book-chapters and one WHO Module. His innovative research has been granted 2 international and one national patents. In recognition of his seminal contribution to the biomedical and health science research, he has been awarded the National Bioscience Award for Career Development, 2011 by the Department of Biotechnology, Government of India and Fulbright-Nehru Senior Research Fellowship, 2012. He is also an elected member of the West Bengal Academy of Science and Technology.

Profile

Publications

For complete bibliography, please see: http://1.usa.gov/21gp5Ff

2019

1. Dasgupta S, Das S, Biswas A, Bhadra RK, Das S. Small alarmones (p)ppGpp regulate virulence associated traits and pathogenesis of Salmonella entericaserovarTyphi.Cellular Microbiology. 2019 Aug;21(8):e13034 Pubmed

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2. 2. Das S, Chowdhury R, Pal A, Okamoto K, Das S. Salmonella Typhi outer membrane protein STIV is a potential candidate for vaccine development against typhoid and paratyphoid fever.Immunobiology. 2019 May;224(3):371-382. Pubmed

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3. Dasgupta N, BK Thakur, A Chakraborty, S Das. Butyrate-induced in vitro colonocyte differentiation network model identifies ITGB1, SYK, CDKN2A, CHAF1A, and LRP1 as the prognostic markers for colorectal cancer recurrence. Nutr Cancer. 2019;71(2):257-271.  Pubmed

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4. Chowdhury R, S Das, A Ta, S Das. Epithelial invasion by Salmonella Typhi using STIV-Met Interaction. Cell Microbiol. 2019 Mar;21(3):e12982.   Pubmed

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2018

1. 1. Thriemer K, A Bobogare, B Ley, CS Gudo, MS Alam, NM Anstey, E Ashley, JK Baird, C Gryseels, E Jambert, M Lacerda, F Laihad, J Marfurt, AP Pasaribu, JR Poespoprodjo, I Sutanto, WR Taylor, C van den Boogaard, KE Battle, L Dysoley, P Ghimire, B Hawley, J Hwang, WA Khan, RNB Mudin, ME Sumiwi, R Ahmed, MM Aktaruzzaman, KR Awasthi, A Bardaji, D Bell, L Boaz, FH Burdam, D Chandramohan, Q Cheng, K Chindawongsa, J Culpepper, S Das et al. Quantifying primaquine effectiveness and improving adherence: a round table discussion of the APMEN Vivax Working Group. Malar J. 2018 Jun 20;17(1):241. Pubmed

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2. Banerjee A, M Lo, Indwar, AK Deb, S Das, B Manna, S Dutta, UK Bhadra, M Bhattacharya, P Okamoto K, M Chawla-Sarkar. Upsurge and spread of G3 rotaviruses in Eastern India (2014-2016): Full genome analyses reveals heterogeneity within Wa-like genomic constellation. Infect Genet Evol. 2018 May 26;63:158-174. Pubmed

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3. Mukherjee SK, Mandal RS, Das S, Mukherjee M. Effect of non-β-lactams on stable variants of inhibitor-resistant TEM β-lactamase in uropathogenic Escherichia coli: implication for alternative therapy. J Appl Microbiol. 2018 Mar;124(3):667-681.  Pubmed

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4. Barik A, Das S. A comparative study of sequence - and structure-based features of small RNAs and other RNAs of bacteria. RNA Biol. 2018 Jan 2;15(1):95-103.  Pubmed

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2017

1. Chowdhury R, H Ilyas, A Ghosh, H Ali, A Ghorai, A Midya, NR Jana, S Das, A. Bhunia. Multivalent Gold nanoparticle-Peptide Conjugates for Targeting Intracellular Bacterial Infections. Nanoscale. 2017 Sep 28;9(37):14074-14093. Pubmed

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2. Jana S, D Patel, S Patel, K Upadhyay, J Thadani, R Mandal, S Das, R Devkar. Anthocyanin rich extract of Brassica oleracea L. alleviates experimentally induced myocardial infarction. PLoS One. 2017 Aug 1;12(8):e0182137 Pubmed

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3. Das S, R Chowdhury, S Ghosh, S Das. A recombinant protein of Salmonella Typhi induces humoral and cell-mediated immune responses including memory responses. Vaccine. 2017 Aug 16;35(35 Pt B):4523-4531. Pubmed

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4. Ta A, BK Thakur, P Dutta, R Sinha, H Koley, S Das. Double-stranded RNA induces cathelicidin expression in the intestinal epithelial cells through phosphatidylinositol 3-kinase-protein kinase Cζ-Sp1 pathway and ameliorates shigellosis in mice. Cell Signal. 2017 Jul; 35:140-153 Pubmed

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5. Sinha R, DR Howlader, A Ta, S Mitra, S Das, H Koley. Retinoic acid pre-treatment down regulates V. cholerae outer membrane vesicles induced acute inflammation and enhances mucosal immunity. Vaccine. 2017 Jun 16; 35(28):3534-3547. Pubmed

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6. Mandal RS, S Panda, S Das. In silico prediction of drug resistance due to S247R mutation of Influenza H1N1 neuraminidase protein. J Biomol Struct Dyn. 2017 Apr 10:1-15. Pubmed

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7. Barman RK, A Mukhopadhyay, S Das. An improved method for identification of small non-coding RNAs in bacteria using support vector machine. Sci Rep. 2017 Apr 6; 7:46070. Pubmed

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8. Barman RK, A Mukhopadhyay, S Das. An improved method for identification of small non-coding RNAs in bacteria using support vector machine. Sci Rep. 2017 Apr 6; 7:46070. Pubmed

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9. Dasgupta N, BK Thakur, A Ta, S Das, G Banik, S Das. Polo-like kinase 1 expression is suppressed by CCAAT/enhancer-binding protein α to mediate colon carcinoma cell differentiation and apoptosis. Biochim Biophys Acta. 2017 Jul; 1861(7):1777-1787. Pubmed

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10. Dutta P, A. Ta, B. K. Thakur, N. Dasgupta, S Das. Biphasic Ccl20 regulation by Toll-like receptor 9 through the activation of ERK-AP-1 and non-canonical NF-κB signaling pathways. Biochim Biophys Acta. 2017 Jan; 1861(1 Pt A):3365-3377. Pubmed

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11. 10. Dasgupta N., B. K. Thakur, A. Ta, P. Dutta, and S. Das. Suppression of Spleen Tyrosine Kinase (Syk) by Histone Deacetylation Promotes, Whereas BAY61-3606, a Synthetic Syk Inhibitor Abrogates Colonocyte Apoptosis by ERK Activation. J Cell Biochem. 2017 Jan; 118(1):191-203. Pubmed

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2016

1. Mandal RS, A. Ta, R. Sinha, N. Theeya, A. Ghosh, M. Tasneem, A. Bhunia, H. Koley, S. Das. 2016. Ribavirin suppresses bacterial virulence by targeting LysR-type transcriptional regulators. Sci Rep. 6:39454. Pubmed

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2. Thakur B. K., N. Dasgupta, A. Ta, and S. Das. 2016. Physiological TLR5 expression in the intestine is regulated by differential DNA binding of Sp1/Sp3 through simultaneous Sp1 dephosphorylation and Sp3 phosphorylation by two different PKC isoforms. Nucleic Acids Res. 2016 Jul 8;44(12):5658-72 Pubmed

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3. Thakur B. K., P. Saha, G. Banik, D. R. Saha, S. Grover, V. K. Batish, and S. Das. 2016. Live and heat-killed probiotic Lactobacillus casei Lbs2 protects from experimental colitis through Toll-like receptor 2-dependent induction of T-regulatory response. Int Immunopharmacol. 36:39-50 Pubmed

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4. Parida S, I. Pal, A. Parekh, B. Thakur, R. Bharti, S. Das, and M. Mandal. 2016. GW627368X inhibits proliferation and induces apoptosis in cervical cancer by interfering with EP4/EGFR interactive signaling. Cell Death Dis.;7:e2154 Pubmed

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5. Saha P, C. Manna, S. Das, and M. Ghosh. 2016. Antibiotic binding of STY3178, a yfdX protein from Salmonella Typhi. Sci Rep. 2016 Feb 19;6:21305 Pubmed

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6. Datta P, S. Das. 2016. Mammalian antimicrobial peptides: promising therapeutic targets against infection and chronic inflammation. Curr. Top. Med. Chem. 16(1):99-129. Pubmed

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2015

1. Das S and B. K. Thakur. 2015. Mucosal immune system of the respiratory tract: regulation of tolerance and immune response. The Pulmo-Face XV(2): 61-70.

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2. Mandal R. S and S. Das. In silico approach towards identification of potential inhibitors of Helicobacter pyloriDapE. J BiomolStructDyn.2015 Jul.33(7):1460-73 Pubmed

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3. Dasgupta N, B. K. Thakur, A. Ta A and S. Das. 2015. Caveolin-1 is transcribed from a hypermethylated promoter to mediate colonocyte differentiation and apoptosis.Exp Cell Res. 2015 Jun 10;334(2):323-36 Pubmed

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4. Banerjee R, S.Das, S.Basak. Similarity of currently circulating H1N1 virus with the 2009 pandemic clone: Viability of an imminent pandemic. Infection, Genetics and Evolution. 2015 Jun; 32:107-112. Pubmed

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5. Chowdhury R, R. S. Mandal, A. Ta and S. Das. An AIL family protein promotes Type Three Secretion System-1 independent invasion and pathogenesis of Salmonella entericaserovar Typhi. Cell Microbiol. 2015 Apr. 17(4):486-503 Pubmed

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6. Nagaraja T, A. Ta, S. Das, R. S. Mandal, O. Chakrabarti, S. Chakrabarti, A. N. Ghosh and S. Das. An Inducible and Secreted Eukaryotic-like Serine/Threonine Kinase of Salmonella Typhi Promotes Intracellular Survival and Pathogenesis. 2015. Infect Immun. 83(2):522-33 Pubmed

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7. Barman R. K., T. Jana, S. Das, S. Saha. 2015. Prediction of intra-species protein-protein interactions in enteropathogens facilitating systems biology study. PLoS One. 10(12):e0145648. Pubmed

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8. Mandal R. S., S. Saha, S. Das. 2015. Metagenomic surveys of gut microbiota. Genomics, Proteomics Bioinformatics 13(3):148-158. Pubmed

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2014

1. Bhowmick S, M. Malar, A. Das, B. K. Thakur, P. Saha, S. Das, H. M. Rashmi, V. K. Batish, S. Grover and S. Tripathy. 2014. Draft Genome sequence of Lactobacillus casei Lbs2. Genome Announc.2(6): e01326-14 Pubmed

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2. Barman RK, S. Saha, S Das. 2014. Prediction of interactions between viral and host proteins using supervised machine learning methods. PLoS ONE. 9(11):e112034 Pubmed

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3. Dhal P. K, R. K Barman, S. Saha and S. Das. 2014. Dynamic Modularity of Host Protein Interaction Networks in Salmonella Typhi Infection. PLoS One. 9(8):e104911 Pubmed

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4. Bhattacherjee A, R.S. Mandal, S. Das, S. Kundu. 2014. Sequence and 3D structure based analysis of TNT degrading proteins in Arabidopsis thaliana. J Mol Model. 20(3):2174. Pubmed

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2013

1. Rajendra Kumar Labala, Santasabuj Das, and Surajit Basak 2013. ASRDb: A comprehensive resource for archaeal stress response genes. Bioinformation. 9: 650-655. Pubmed

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2012

1. Dasgupta A., R. Banerjee, S. Das and S. Basak. 2012. Evolutionary perspective on the origin of Haitian cholera outbreak strain. J Biomol Struct Dyn. 30: 338-46. Pubmed

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2. Banerjee R, A. Roy, F. Ahmad, Das S, S. Basak. 2012. Evolutionary patterning of hemagglutinin gene sequence of 2009 H1N1 pandemic. J Biomol Struct Dyn. 29: 733-742. Pubmed

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2011

1. Ghosh S., K. Chakraborty, T. Nagaraja, S. Basak, H. Koley, S. Dutta, U. Mitra and S. Das. 2011. An adhesion protein of Salmonella enterica serovar Typhi is required for pathogenesis and potential target for vaccine development.
   Proc Natl Acad Sci U S A. 108: 3348-3353. Pubmed

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2010

1. Roy N, S. Barman, A. Ghosh, A. Pal, K. Chakraborty, S. Das, D. R. Saha, S. Yamasaki and H. Koley. 2010. Immunogenicity and protective efficacy of Vibrio cholerae outer membrane vesicles in rabbit model. FEMS Immunol Med Microbiol. 60: 18-27. Pubmed

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2. Bhadra R. K., S. Das and G. B. Nair. 2010. Immunological basis for Immunization: Cholera. WHO/IVB/ISBN 978 92 4 159974 0, pp.1-31. Pubmed

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2009

1. Sinha, N. K., A. Roy, B. Das, S. Das and S. Basak. 2009. Evolutionary complexities of swine flu H1N1 gene sequences of 2009. Biochem Biophys Res Commun. 390: 349-51. Pubmed

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2. Chakraborty, K., P. C. Maity, A. K. Sil, Y. Takeda and S. Das. 2009. cAMP stringently regulates human cathelicidin antimicrobial peptide expression in the mucosal epithelial cells by activating cAMP-response element-binding ptotrin, AP-1, and Inducible cAMP early repressor. J Biol Chem. 284:21818-21827. Pubmed

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3. Basak, S., R. Banerjee, I. Mukherjee, S. Das. 2009. Influence of domain architecture and codon usage pattern on the evolution of virulence factors of Vibrio cholerae. Biochem Biophys Res Commun. 379: 803-805. Pubmed

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2008

1. Basak, S., I. Mukherjee, M. Choudhury and S. Das. 2008. Unusual codon usage bias in low expression genes of Vibrio cholerae. Bioinformation. 3: 213-217. Pubmed

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2. Chakraborty, K., S. Ghosh, H. Kole, A. K. Mukhopadhyay, T. Ramamurthy, D. R. Saha, D. Mukhopadhyay, S. Roychowdhury, T. Hamabata, Y. Takeda and S. Das. 2008. Bacterial exotoxins downregulate cathelicidin (hCAP18/LL37) and human defensin 1 (HBD-1) expression in the intestinal epithelial cells. Cell Microbiol. 10: 2520-2537. Pubmed

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3. Chakraborty, K., S. Ghosh, H. Kole, A. K. Mukhopadhyay, T. Ramamurthy, D. R. Saha, D. Mukhopadhyay, S. Roychowdhury, T. Hamabata, Y. Takeda and S. Das. 2008. Bacterial exotoxins downregulate cathelicidin (hCAP18/LL37) and human defensin 1 (HBD-1) expression in the intestinal epithelial cells. 2008. (In Press). Pubmed

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2007

1. Van Acker, G. J., G. Perides, E. R. Weiss, S. Das, P. N. Tsichlis and M. L. Steer. 2007. Tumor progression locus-2 is a critical regulator of pancreatic and lung inflammation during acute pancreatitis. J. Biol Chem. 282: 22140-9. Pubmed

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2006

1. Ghosh, A., D. R. Saha, K. M. Hoque, M. Asakuna, S. Yamazaki, H. Koley, S. Das, M. K. Chakraborty and A. Pal. 2006. Enterotoxigenicity of 45kDa matured and 35kDa processed forms of hemagglutinin protease purified from a cholera toxin gene negative Vibrio cholerae non-O1non-O139 strain. Infect Immun. 74: 2937-46. Pubmed

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2. Eliopoulos, A. G., S. Das and P. N. Tsichlis. 2006. The tyrosine kinase Syk regulates TPL2 activation signals. J. Biol Chem. 281: 1371-80. Pubmed

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2005

1. Das, S., J. Cho, I. Lambertz, M. A. Kelliher, A. G. Eliopoulos, K. Du and P. N. Tsichlis. 2005. Tpl2/Cot Signals Activate ERK, JNK, and NF-?B in a Cell-type and Stimulus-specific Manner. J. Biol Chem. 280: 23748-57. Pubmed

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2002

1. Das, S., J. H. Lin, J. Papamatheakis, Y. Sykulev and P. N. Tsichlis. 2002. Differential splicing generates Tvl-1/RFXANK isoforms with different functions. J. Biol Chem. 277: 45172-80. Pubmed

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Books/ Book Chapters/ Training Modules:

1. Mandal RS, S. Das. 2017. In Silico Approaches Toward Combating Antibiotic Resistance. In Arora G, sajid A, Kalia VC (eds), Drug Resistance in Bacteria, Fungi, Malaria, and Cancer. Springer, pp.577-593.

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2. Dutta P and S Das. 2015. Antimicrobial peptides and infectious Diseases. In Mendez-Vilas A (ed), The Battle against Microbial Pathogenesis: Basic Science and Educational Programmes, 5th series. Badazgoz Spain, Formatex Research Centre.

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3. 3. Bhadra R. K., S. Das and G. B. Nair. 2010. Immunological basis for Immunization: Cholera. WHO/IVB/ISBN 978 92 4 159974 0, pp.1-31.

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Patents Granted

1. Das S, Ghosh S. A novel salmonella typhi protein as subunit vaccine. (Patent No. 283894; Pub Date: 09.09.2011).
   

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2. Das S, Bhunia A. A peptide to neutralize lipopolysaccharide molecule. (Pub No. WO 2016 132377 A1; Pub Date: 25/08/2016).
   

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3. Koley H, Mitra S, Das S, Chakrabarti MK. A multi-serotype outer membrane vesicles (momv) of Shigellae as a novel candidate vaccine. (Pub No. WO/2014/192031; Pub Date: 04.12.2014).
   

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