National Institute of Cholera and Enteric Diseases

Indian Council of Medical Research
Department of Health Research, Ministry of Health and Family Welfare, Government of India
WHO Collaborating Centre for Research and Training on Diarrhoeal Diseases

Facilities

Facilities

Library

To support the divergent and vigorous academic activity of the Institute, the NICED library is fully geared to meet the needs of the scientists and other research workers. NICED Library has undergone several phases of modernization since 1988. It began its journey with a mere collection of a few books in a small room. Read more...

Animal Facility

The Animal Facility of NICED consists of two units. The old facility is present at the premises of S.C.PAL-Building. This has undergone phase wise modernization and at present it looks like a new version of old Animal House. The new facility is developed at the JICA-building premises of NICED with modern technological outfits. As a whole, the entire facility is updated to cater the need of Scientific Research of this Institute.

Scientists of National Institute of Cholera and Enteric Diseases (NICED) are working on different aspects of diarrheal diseases with special emphasis on development of vaccines against enteric diseases. Our Institute also raises and supplies antisera for detection and serotyping of Vibrio cholerae strains. NICED Animal Facilities division is engaged in care, breeding and management of animals required for studies conducted by researchers of the Institute.

The Micro Ventilated Cage System: Modern animal house facility at NICED was developed in collaboration with JICA project. This facility houses special animal cage system in which the air flow is maintained through the enclosures by a pressure differential system between the cage chamber and the exhaust chamber. This is accomplished by maintaining the cage chamber at a positive pressure with respect to the enclosures and maintaining the exhaust chamber at a negative pressure with respect to the enclosures. Both the input and the exhausted air are sterilized by various devices in the path of the ventilation stream. Purpose is to keep the animal germ free. The optimum humidity and temperature of the cage chamber is maintained.

Surgical room is equipped with modern instruments like radio frequency operated surgical knife, anesthesia maintaining ventilation device, Vacuumed operated animal hair clipping machine etc which are used during operation and immunization to give minimum pain to the animal.

Successful management of our animal resource facilities involves effective utilization of manpower to achieve the goal of comprehensive animal care. We have trained and experienced staff to look after the animals. This facility has been given the responsibilities of production and maintenance of laboratory animals for research.

Recently we are having the following laboratory animal species and strains: 

Species Strains Status
Rabbit New Zealand White Outbred
Guinea Pig English Albino Outbred
Hamster Golden hamster Outbred
Sheep Indian Sheep Outbred
Rat Wister Inbred
Mice Swiss Outbred
Mice BALB/C Inbred
Mice C57BL/6 Inbred
Institutional Animal Ethics Committee (IAEC) has been instituted for the purpose of control and supervision of research on experimental Control and supervision of experiments on animal (CPCSEC) also looks into all aspects of research on animals including ethical issues, preventive medicine program for disease control. It also advises to the researchers on the varies aspect of animals experiments including the experimental design, pre and post operative care after surgical operation.

Biosafety Level-3 Laboratory (BSL3)

In wake of increasing threat of highly pathogenic viral infections such as Avian Influenza, Ministry of Health and Family Welfare provided funds in 2006 for setting up a BSL3 laboratory at NICED to cater to the Eastern states of India. A modular prefabricated BSL3 laboratory was installed and inaugurated at NICED in 2007 in the ID and BG hospital Campus by Director General, ICMR in the presence of Additional Chief Secretary, Govt of West Bengal and other dignitaries from the State Health Department. BSL-3 is suitable for work with infectious agents which may cause serious or potentially lethal diseases as a result of exposure even through aerosol. During the Avian Influenza outbreak in poultry in West Bengal during January-February 2008 and Influenza A/H1N1/ 2009 pandemic during July-Dec 2009 all suspected human samples from West Bengal and neighboring states were handled and analyzed by Real time PCR in the BSL3 laboratory. Subsequently the lab has also been utilized during INFA/H1n!/ 2009 outbreak during 2015 for providing laboratory services to the state. This facility is under the Division of Virology.

Instrumentation

The Institute has a central instrumentation facility located in the 3rd floor of S. C. Pal Building. Due to recent expansion, another set up will be coming up soon in the 2nd floor of JICA building. The central facility is equipped with high-speed centrifuges, ultracentrifuges, spectrophotometer, sonicator, and shakers for large bacterial cultures. This facility is being upgraded time to time. Recently, a large number of sophisticated instruments have been procured and these are placed under scientists for proper maintenance and utilization. These include FPLC, HPLC, LC-MS, calorimeters like ITC and DSC, FACS, confocal microscope, electron microscope, real-time PCR and automated DNA sequencer. The instruments are available for use mainly by our scientists and our collaborators.

Flow Cytometry Core Facility:

NICED established its Flow cytometry(Fluorescence activated Cell Sorting or FACS) as central Instrument facility in 2010. Flow cytometry is a laser-based, biophysical technology employed in cell counting, cell sorting, biomarker detection and protein engineering, by suspendingcells in a stream of fluid and passing them by an electronic detection apparatus. It allows simultaneous multiparametric analysis of the physical and chemical characteristics of up to thousands of particles per second. It is routinely used in the diagnosis of health disorders, especially blood cancers/ HIV etc. NICED facility has FACSAria II flow cytometer with 9 colour (2 near ultra violet, 5 blue and 2 red) three laser system with 375nm near UV, 488nm blue and 633nm red laser. The lasers are contained and the laser energy is rendered within the instrument structure making it completely safe during its functioning. No special work area safety is required. Structurally, it has three main components - (i) fluidics cart (pumps provide 5 - 75 psi pressure to accommodate a variety of cell sorting), (ii) optics system (comprising of lasers, fiber optics, optic filters and photomultiplier tubes (PMTs)) and (iii) cytometer electronics (converting optical signal to digital signal). This set up is highly compatible for both the purposes of analysis and sorting

Electron Microscopy

The electron microscopes are used primarily for research and diagnosis. The techniques in routine use are negative staining analysis, Kleinschmidt's protein monolayer technique of DNA, partial denaturation mapping and heteroduplex analysis of DNA, protein-free spreading methods of DNA and RNA, immunoelectron microscopy, ultramicrotomy (both cryo and room temperature), cryoelectron microscopy, single particle analysis and tomography for three-dimensional image reconstruction, environmental scanning electron microscopy and atomic force microscopy.

The division has the following equipments:

  1. FEI Tecnai 12 BioTwin Transmission Electron Microscope with Gatan cryostage (cryoTEM) and Explore 3D Tomography package.
  2. FEI Quanta 200 Environmental Scanning Electron Microscope (eSEM)
  3. Shimadzu SPM-9500J3 Atomic Force Microscope (AFM) with liquid cell.
  4. Leica Ultracut UCT Ultramicrotome with EMFCS cryo attachment
  5. Leica EMKMR2 Knifemaker
  6. Leica EMSTAIN Autostainer
  7. Leica EMTRIM Trimmer
  8. Leica EM CPC universal cryo-workstation
  9. Polaron CPD7501 Critical Point Drier (CPD)
  10. Polaron Sputter Coater
  11. JEOL JEE-400 High Vacuum Evaporator (shadow caster)
  12. JEOL HDT-400 Glow Discharge apparatus
  13. Gatan Model 655 Dry Pumping Station
  14. Iwatani NL-50 Liquid Nitrogen Generator

GTPR

Bioinformatics

The Biomedical Informatics Centre (BIC) of National Institute of Cholera and Enteric Diseases, Kolkata was established in July, 2006. This is the one of the 20 centres created by the Indian Council of Medical Research (ICMR), Govt. of India with a mission to ensure balanced and integrated development of information dissemination, application development and R&D in biomedical informatics.

© 2016   NICED,  All rights reserved.   |   Designed by :   Braindrops