I, Dr. Nabendu Sekhar Chatterjee, Scientist D, was born on 2 September 1965 and joined National Institute of Cholera and Enteric Diseases, ICMR, on 17 August 2001. I did B.Sc in Chemistry from Presidency College (Calcutta University) in 1988; M.Sc. in Biochemistry from Calcutta University in 1990; and Ph.D. in Chemistry from the University of Nebraska-Lincoln, USA in 1997. Specialization is molecular biology with special emphasis on molecular characterization of translation-related gene products and studying transcriptional regulation of the related genes. Visiting postdoctoral researcher in the University of California-Davis, USA (1997-1998); Post graduate researcher in the University of California-Irvine, USA during 1998-2001 with experience the molecular regulation of water-soluble vitamin transporters.
My lab is currently interested in different aspects of interaction between host and pathogen. The host-pathogen interaction research involves understanding molecular biology of bacterial pathogens and the mechanisms by which they interact with hosts. Two bacterial pathogens under investigation are Vibrio cholerae, and enterotoxigenic Escherichia coli, which are the prevalent bacterial pathogens for watery diarrhea in developing countries. We have undertaken different projects to characterize different adhesins/colonization factors and study the regulation of their gene expression. We also try to identify and characterize the host receptors involved in bacterial colonization, study host host receptor and pathogen's colonization factors' interaction and understand the molecular mechanisms of colonization. We further aim to look into host response triggered by the colonization factors from V. cholerae and ETEC and induction of host signal transduction pathways by these pathogens during colonization. I am member of the following societies.
Chourashi R, Das S, Dhar D, Okamoto K, Mukhopadhyay AK,
Chatterjee NS. Chitin-induced T6SS in Vibrio cholerae is
dependent on ChiS activation. Microbiology. 2018 May;
Chourashi R, Mondal M, Sinha R, Debnath A, Das S, Koley H,
Chatterjee NS. Role of a sensor histidine kinase ChiS of
Vibrio cholerae in pathogenesis. Int J Med Microbiol. 2016
Tapader R, Bose D, Basu P, Mondal M, Mondal A, Chatterjee NS,
Dutta P, Basu S, Bhadra RK, Pal A. Role in proinflammatory
response of YghJ, a secreted metalloprotease from neonatal
septicemic Escherichia coli. Int J Med Microbiol. 2016
Mukherjee A, Ganguly S, Chatterjee NS, Banerjee KK.
Vibrio cholerae hemolysin: The β-trefoil domain is required
for folding to the native conformation. Biochem Biophys Rep.
2016 Sep 22;8:242-248.
Mandal S, Chatterjee NS. Vibrio cholerae GbpA
elicits necrotic cell death in intestinal cells. J Med Microbiol.
Debnath A, Sabui S, Wajima T, Hamabata T, Banerjee R,
Chatterjee NS. Functional Role of N- and C-Terminal Amino
Acids in the Structural Subunits of Colonization Factor CS6
Expressed by Enterotoxigenic Escherichia coli. J
Bacteriol. 2016 Apr 14;198(9):1429-41.
Mondal A, Tapader R, Chatterjee NS, Ghosh A, Sinha R,
Koley H, Saha DR, Chakrabarti MK, Wai SN, Pal A. Cytotoxic and
Inflammatory Responses Induced by Outer Membrane
Vesicle-Associated Biologically Active Proteases from Vibrio
cholerae. Infect Immun. 2016 Apr 22;84(5):1478-90.
Mondal M, Chatterjee NS. Role of Vibrio cholerae
exochitinase ChiA2 in horizontal gene transfer. Can J Microbiol.
9. Sabui S, Debnath A, Ghosal A, Wajima T, Hamabata T,
Ramamurthy T, Ghosh AN, Basak S, Chatterjee NS.
Characterization of oligomeric assembly of colonization factor
CS6 from enterotoxigenic Escherichia coli. Microbiology.
8. Debnath A, Wajima T, Sabui S, Hamabata T, Ramamurthy T, Chatterjee NS. Two specific amino acid variations in
colonization factor CS6 subtypes of enterotoxigenic
Escherichia coli results in differential binding and
pathogenicity. Microbiology. 2015 Apr;161(Pt 4):865-74.
Mondal, M., D. Nag, H. Koley, D. R. Saha and N. S. Chatterjee. 2014.The Vibrio choleraeextracellular chitinase ChiA2
is important for survival and pathogenesis in thehost intestine. PLoS One.9(9):e103119
Wajima, T., S. Sabui, S. Kano, T. Ramamurthy, N. S. Chatterjee
and T. Hamabata. 2013. Entire sequence of the colonization factor
coli surface antigen 6-encoding plasmid pCss165 from an enterotoxigenic
Escherichia coli clinical isolate. Plasmid 70: 343-352.
Ghosal, A., N. S. Chatterjee, T. Chou and H. M. Said. 2013.
Enterotoxigenic Escherichia coli (ETEC) infection and intestinal thiamin
uptake: studies with intestinal epithelial Caco-2 monolayers. Am J
Physiol Cell Physiol. 305: C1185-1191.
Wong, E., G. Vaaje-Kolstad, A. Ghosh, R. Hurtado-Guerrero, P. V. Konarev, A. F. Ibrahim, D. I. Svergun, V. G. Eijsink, N. S. Chatterjee
and D. M. van Aalten. 2012. The Vibrio cholere colonization
factor GbpA possesses a modular structure that governs binding to different host surfaces. PLoS Pathog. 8: e1002373.
Ghosh, A., A. Choudhury, A. Das,N. S. Chatterjee, T. Das, R. Chowdhury, K. Panda, R. Banerjee and I. B. Chatterjee.
2012. Cigarette smoke induces p-benzoquinone-albumin adduct
in blood serum: Implications on structure and ligand binding properties.Toxicology292:78-89
Sabui, S., S. Dutta, A. Debnath, A. Ghosh, T. Hamabata, K. Rajendran, T. Ramamurthy, J.P. Nataro, D. Sur, M.M. Levine and N.S. Chatterjee.
2012. Real-time PCR-based mismatch amplification mutation assay for specific detection of CS6-expressing allelic variants of enterotoxigenic
Escherichia coli and its application in assessing diarrheal cases and asymptomatic controls. J Clin Microbiol. 50: 1308-1312.
Chatterjee, R., A. Ghosal, S. Sabui
and N. S. Chatterjee. 2011. Three dimensional modelling of
C-terminal loop of CssA subunit in CS6 of Enterotoxigenic Escherichia
coli and its interaction with the 70 KDa domain of Fibronectin.
Bioinformation 6: 307-310.
Wajima T., S. Sabui, M. Fukumoto, S.
Kano, T. Ramamurthy, N. S. Chatterjee and T. Hamabata. 2011.
Enterotoxigenic Escherichia coli CS6 gene products and their
roles in CS6 structural protein assembly and cellular adherence.
Microbial Pathogen. 51: 243-249.
Sabui, S., A. Ghosal, S. Dutta, A.Ghosh, T. Ramamurthy, J.P. Nataro, T. Hamabata and N. S.Chatterjee. 2010.
Allelic variation in colonization factor CS6 of enterotoxigenic Escherichia coli isolated
from patients with acute diarrhoea and controls. J Med Microbiol. 59: 770-779.
Bhowmick, D.C., B. Bal, N. S. Chatterjee, A. N. Ghosh and S. Pal. 2009. A low-GC Gram-positive Thermoanaerobacter-like
bacterium isolated from an Indian hot spring contains Cr(VI) reduction activity
both in the membrane and cytoplasm. J. Appl. Microbiol. 106: 2006-2016.
Ghosal, A., R. Bhowmick, R. Banerjee, S. Ganguly, S.
Yamasaki, T. Ramamurthy, T. Hamabata and N. S. Chatterjee. 2009.
Characterization and studies of the cellular interaction of native
colonization factor CS6 purified from a clinical isolate of
enterotoxigenic Escherichia coli. Infect. Immun. 77: 2125-2135.
Bhowmick, R., A. Ghosal, B. Das, H. Koley, D. R. Saha,
S. Ganguly , R. K. Nandy, R. K. Bhadra and N. S. Chatterjee. 2008.
Intestinal adherence of Vibrio cholerae involves a coordinated
interaction between colonization factor GbpA and mucin. Infect Immun.
Ghosal, A., R. Bhowmick, R. K. Nandy, T. Ramamurthy and
N. S. Chatterjee. 2007. PCR-based identification of common
colonization factor antigens of enterotoxigenic Escherichia coli.
J Clin Microbiol. 45: 3068-3071.
Bhowmick, R., A. Ghosal, and N. S. Chatterjee. 2007.
Effect of environmental factors on expression and activity of chitinase
genes of vibrios with special reference to Vibrio cholerae. J. Appl. Microbiol. 103: 97-108.
Sen, A., N. S. Chatterjee, M. A. Akbar, N. Nandi and P. Das. 2007. The 29-kilodalton thiol-dependent peroxidase of
Entamoeba histolytica is a factor involved in pathogenesis and survival
of the parasite during oxidative stress. Eukaryot. Cell. 6:
Akbar, M. A., N. S. Chatterjee, P. Sen, A. Debnath, A.
Pal, T. Bera and P. Das. 2004. Genes induced by a high-oxygen
environment in Entamoeba histolytica. Mol. Biochem. Parasitol.
Ray, A., N. S. Chatterjee, S. K. Bhattacharya and T. Biswas. 2003. Porin of Shigella dysenteriae enhances mRNA
levels for Toll-like receptor 2 and MyD88, up-regulates CD80 of murine
macrophage, and induces the release of interleukin-12. FEMS Immunol.Med. Microbiol. 39: 213-219.
Saha, M.R., A. Palit, N. S. Chatterjee, P. Dutta, U.
Mitra and S. K. Bhattacharya. 2003. A prospective study of phage
types biotypes of Salmonella enterica serotype Typhi isolated
from hospitalized children in Kolkata, India. Indian J. Med. Res. 117:
Subramanian, V. S., N. S. Chatterjee and H. M. Said. 2003.
Folate uptake in the human intestine: promoter activity and effect of
folate deficiency. J. Cell. Physiol. 196: 403-408.
Dey, S., V. S. Subramanian, N. S. Chatterjee, S. A. Rubin
and H. M. Said. 2002. Characterization of the 5' regulatory region
of the human sodium-dependent multivitamin transporter, hSMVT. Biochim.
Biophys. Acta. 1574: 187-192.
Chatterjee, N. S., S. A. Rubin and H. M. Said. 2001.
Molecular characterization of the 5' regulatory region of rat
sodium-dependent multivitamin transporter gene. Am. J. Physiol. Cell
Physiol. 280: C548-C555.
Said, H. M., N. S. Chatterjee, R. U. Haq, V. S. Subramanian,
A. Ortiz, L. H. Matherly, F. M. Sirotnak, C. Halsted and S. A. Rubin.
2000. Adaptive regulation of intestinal folate uptake: effect of
dietary folate deficiency. Am. J. Physiol. Cell Physiol. 279:
Chatterjee, N. S., C. K. Kumar, A. Ortiz, S. A. Rubin and
H. M. Said. 1999. Molecular mechanism of the intestinal biotin
transport process. Am. J. Physiol. Cell Physiol. 277: C605-613.
Said, H. M., A. Ortiz, C. K. Kumar, N. S. Chatterjee, P. K.
Dudeja and S. Rubin. 1999. Transport of thiamine in human
intestine: mechanism and regulation in intestinal epithelial cell model
Caco-2. Am. J. Physiol. Cell Physiol. 277: C645-651.
Chatterjee, M., N. S. Chatterjee, R. Datta, B. Datta and
N. K. Gupta. 1998. Expression and activity of p67 are induced
during heat shock. Biochem. Biophys. Res. Commun. 249: 113-117.
Saha, D., S. Wu, A. Bose, N. S. Chatterjee, A. Chakraborty,
M. Chatterjee and N. K. Gupta. 1997. Viral infection. II. Hemin
induces overexpression of p67 as it partially prevents appearance of an
active p67-deglycosylase in baculovirus-infected insect cells. Arch.
Biochem. Biophys. 342: 373-382.
Gupta, S., A. Bose, N. S. Chatterjee, D. Saha, S. Wu, N. K.
Gupta. 1997. p67 transcription regulates translation in
serum-starved and mitogen-activated KRC-7 cells. J. Biol Chem. 272:
Chatterjee, N. S., C. Zou, J. C. Osterman and N. K. Gupta.
1997. Cloning and characterization of the promoter region of a
gene encoding a 67-kDa glycoprotein. J. Biol Chem. 272:
Gupta, S., S. Wu, N. S. Chatterjee, J. Ilan , J. Ilan, J. C. Osterman and N. K. Gupta. 1995. Regulation of an eukaryotic
initiation factor-2 (eIF-2) associated 67 kDa glycoprotein (p67) and its
requirement in protein synthesis. Gene Expr. 5: 113-122.
Wu, S., S. Gupta, N. S. Chatterjee, R. E. Hileman, T. G.
Kinzy, N. D. Denslow, W. C. Merrick, D. Chakrabarti, J. C. Osterman and
N. K. Gupta. 1993. Cloning and characterization of complementary
DNA encoding the eukaryotic initiation factor 2-associated 67-kDa
protein (p67). J. Biol Chem. 268: 10796-10801.