National Institute of Cholera and Enteric Diseases

Indian Council of Medical Research
Department of Health Research, Ministry of Health and Family Welfare, Government of India
WHO Collaborating Centre for Research and Training on Diarrhoeal Diseases

NICED : Scientists

Dr. Nabendu Sekhar Chatterjee

Dr. Nabendu Sekhar Chatterjee

I, Dr. Nabendu Sekhar Chatterjee, Scientist D, was born on 2 September 1965 and joined National Institute of Cholera and Enteric Diseases, ICMR, on 17 August 2001. I did B.Sc in Chemistry from Presidency College (Calcutta University) in 1988; M.Sc. in Biochemistry from Calcutta University in 1990; and Ph.D. in Chemistry from the University of Nebraska-Lincoln, USA in 1997. Specialization is molecular biology with special emphasis on molecular characterization of translation-related gene products and studying transcriptional regulation of the related genes. Visiting postdoctoral researcher in the University of California-Davis, USA (1997-1998); Post graduate researcher in the University of California-Irvine, USA during 1998-2001 with experience the molecular regulation of water-soluble vitamin transporters.

My lab is currently interested in different aspects of interaction between host and pathogen. The host-pathogen interaction research involves understanding molecular biology of bacterial pathogens and the mechanisms by which they interact with hosts. Two bacterial pathogens under investigation are Vibrio cholerae, and enterotoxigenic Escherichia coli, which are the prevalent bacterial pathogens for watery diarrhea in developing countries. We have undertaken different projects to characterize different adhesins/colonization factors and study the regulation of their gene expression. We also try to identify and characterize the host receptors involved in bacterial colonization, study host host receptor and pathogen's colonization factors' interaction and understand the molecular mechanisms of colonization. We further aim to look into host response triggered by the colonization factors from V. cholerae and ETEC and induction of host signal transduction pathways by these pathogens during colonization. I am member of the following societies.

General Information
Name Dr. Nabendu Sekhar Chatterjee
Designation Scientist F
Date of joining ICMR 17th August 2001
Date of joining present post September 01, 2015
Discipline Biochemistry
Division Biochemistry
Specialization Biochemistry and Molecular Biology
Email : chatterjeens.niced@gov.in , nschatterjee@rediffmail.com
Academic Qualification  
Graduation Graduation: B.Sc (Chemistry Hons.)
Post Graduation M.Sc (Biochemistry)
Doctoral Ph.D (Chemistry)

Profile

Research Experience

 I received Ph.D. in 1997 from the University of Nebraska-Lincoln, USA in 1997. My specialization was molecular biology with special emphasis on molecular characterization of translation-related gene products and studying transcriptional regulation of the related genes. I gained experience in biochemical characterization of proteins a visiting postdoctoral researcher in the University of California-Davis, USA (1997-1998). As a post graduate researcher in the University of California-Irvine, USA (1998-2001), my experiencewas on understanding molecular regulation of water-soluble vitamin transporters. I joined ICMR-NICED in August 2001 as Scientist C, where I started research on enteric diseases. Since then my research is focused on host-pathogen interaction. I was promoted to Scientist D in 2006, Scientist E in 2010 and Scientist F in 2015. My experience is to characterize the microbial proteins in relation to structure and pathogenesis of enteric diseases and host response and study the regulation of their gene expression.

Research Interests

My lab is primarily focuses on in-depth understanding of the molecular mechanisms of host pathogen interaction using biochemical and biophysical approaches. The molecules of interest are Vibrio choleraechitinases, chitin-binding proteins, their regulators and colonization factors of enterotoxigenicEscherichia coli (ETEC). Knowledge generated has already begun to provide a greater understanding of the complexity of bacterial pathogenesis and aim to translate the knowledge in developing therapeutic intervention strategies against enteric infections in near future. Further, know-how gathered is being applied to establish molecular tools for detection of virulence markers in pathogenic strains.

Membership/ Fellow of Professional Societies/ Associations

  1. Society of Biological Chemists : Life Member
  2. The Indian Science Congress Association : Life member
  3. Association of Microbiologists of India : Life Member
  4. American Society for Microbiology : Member

Awards

  • Recipient of Fulbright-Nehru Senior Research Fellowship award 2011-2012.

Projects

Extramural

  1. Molecular Aspects of a 47-kDa N-acetyl-D-Glucosamine specific Vibrio cholerae Adhesin (as PI) – Council of Scientific and Industrial Research, Govt. of India (2004-2007)
  2. Studies on high oxygen-induced genes in E. histolytica: role in survival and pathogenicity of parasite (as Co PI) – Department of Biotechnology, Govt. of India (2005-2008)
  3. Studies of different domains of colonization factor CS6 of enterotoxigenic Escherichia coli in search for a probable subunit vaccine candidate (as PI) – Okayama University Project, Govt. of Japan (2007-till date)
  4. Molecular mechanism of enterotoxigenic Escherichia coli adherence in the intestine: host-pathogen relationship (as PI) –Department of Atomic Energy, Govt. of India (2008- 2011)
  5. Studies of different domains of colonization factor CS6 of enterotoxigenic Escherichia coli in search for a probable subunit vaccine candidate-Phase II (as PI) – Okayama University Project, Govt. of Japan (2010-2015)
  6. Host intestinal response induced by Vibrio Cholerae chitin-binding protein GbpA and the subsequent effect on the pathogen (as PI) – Council of Scientific and Industrial Research, Govt. of India (2010-2013)
  7. Vibrio cholerae chitinases in natural competence and pathogenesis in host and environment-Phase III (as PI) – Okayama University Project, Govt. of Japan (2015-2020) 

Intramural

  1. Detection and characterization of different colonization factors for molecular typing of enterotoxigenic Escherichia coli (2004-2009)
  2. Molecular characterization of a Vibrio cholerae secreted chitinase (2008-2011)
  3. Interplay between colonization factor CS6 of enterotoxigenic Escherichia coli and the host intestinal cells (2010-2015)
  4. Molecular typing of Enterotoxigenic Escherichia coli. (2015-2016)
  5. Studies on expression, distribution and regulation of prevalent virulence factors of enterotoxigenic Escherichia coli. (2016-2019)

Students

  • JRF
    • Mr. DebjyotiBhakat
    • Mr. Suman Das
  • SRF
    • Ms.Rhishita Chourashi

Awards

  • Recipient of Fulbright-Nehru Senior Research Fellowship 2011-2012

Travel to foreign countries to attend conference/ meetings

  1. Asian–African Research Forum held in Tokyo, Japan during January 23-24, 2013.
    Title of the presentation: Colonization factor CS6 of enterotoxigenicEscherichia coli:Functional heterogeneity due to sequence variation among CS6 genes.
  2. 5th Congress of the European Microbiologists (FEMS 2013) held in Leipzig, Germany during July 23-26, 2013.
    Title of the presentation: Role of allelic variants of the colonization factor CS6 of enterotoxigenicEscherichia coli in pathogenesis
  3. 14th Congress of the international Union of Microbiologists (IUMS 2014) held in Montreal, Canada during July 27-August 1, 2014.
    Title of the presentation: Assembly of colonization factor CS6 of enterotoxigenic Escherichia coli: Role of specific residues.

Publications

2016

  1. Chourashi R, Mondal M, Sinha R, Debnath A, Das S, Koley H, Chatterjee NS. Role of a sensor histidine kinase ChiS of Vibrio cholerae in pathogenesis. Int J Med Microbiol. 2016 Dec;306(8):657-665.  Pubmed
  2. Tapader R, Bose D, Basu P, Mondal M, Mondal A, Chatterjee NS, Dutta P, Basu S, Bhadra RK, Pal A. Role in proinflammatory response of YghJ, a secreted metalloprotease from neonatal septicemic Escherichia coli. Int J Med Microbiol. 2016 Nov;306(7):554-565.  Pubmed
  3. Mukherjee A, Ganguly S, Chatterjee NS, Banerjee KK. Vibrio cholerae hemolysin: The β-trefoil domain is required for folding to the native conformation. Biochem Biophys Rep. 2016 Sep 22;8:242-248. Pubmed
  4. Mandal S, Chatterjee NS. Vibrio cholerae GbpA elicits necrotic cell death in intestinal cells. J Med Microbiol. 2016 Aug;65(8):837-47.  Pubmed
  5. Debnath A, Sabui S, Wajima T, Hamabata T, Banerjee R, Chatterjee NS. Functional Role of N- and C-Terminal Amino Acids in the Structural Subunits of Colonization Factor CS6 Expressed by Enterotoxigenic Escherichia coli. J Bacteriol. 2016 Apr 14;198(9):1429-41.  Pubmed
  6. Mondal A, Tapader R, Chatterjee NS, Ghosh A, Sinha R, Koley H, Saha DR, Chakrabarti MK, Wai SN, Pal A. Cytotoxic and Inflammatory Responses Induced by Outer Membrane Vesicle-Associated Biologically Active Proteases from Vibrio cholerae. Infect Immun. 2016 Apr 22;84(5):1478-90.   Pubmed
  7. Mondal M, Chatterjee NS. Role of Vibrio cholerae exochitinase ChiA2 in horizontal gene transfer. Can J Microbiol. 2016 Mar;62(3):201-9.   Pubmed
  8. 9. Sabui S, Debnath A, Ghosal A, Wajima T, Hamabata T, Ramamurthy T, Ghosh AN, Basak S, Chatterjee NS. Characterization of oligomeric assembly of colonization factor CS6 from enterotoxigenic Escherichia coli. Microbiology. 2016 Jan;162(1):72-83.   Pubmed

2015

  1. 8. Debnath A, Wajima T, Sabui S, Hamabata T, Ramamurthy T, Chatterjee NS. Two specific amino acid variations in colonization factor CS6 subtypes of enterotoxigenic Escherichia coli results in differential binding and pathogenicity. Microbiology. 2015 Apr;161(Pt 4):865-74.  Pubmed

2014

  1. Mondal, M., D. Nag, H. Koley, D. R. Saha and N. S. Chatterjee. 2014.The Vibrio choleraeextracellular chitinase ChiA2 is important for survival and pathogenesis in thehost intestine. PLoS One.9(9):e103119

2013

  1. Wajima, T., S. Sabui, S. Kano, T. Ramamurthy, N. S. Chatterjee and T. Hamabata. 2013. Entire sequence of the colonization factor coli surface antigen 6-encoding plasmid pCss165 from an enterotoxigenic Escherichia coli clinical isolate. Plasmid 70: 343-352. Pubmed
  2. Ghosal, A., N. S. Chatterjee, T. Chou and H. M. Said. 2013. Enterotoxigenic Escherichia coli (ETEC) infection and intestinal thiamin uptake: studies with intestinal epithelial Caco-2 monolayers. Am J Physiol Cell Physiol. 305: C1185-1191. Pubmed

2012

  1. Wong, E., G. Vaaje-Kolstad, A. Ghosh, R. Hurtado-Guerrero, P. V. Konarev, A. F. Ibrahim, D. I. Svergun, V. G. Eijsink, N. S. Chatterjee and D. M. van Aalten. 2012. The Vibrio cholere colonization factor GbpA possesses a modular structure that governs binding to different host surfaces. PLoS Pathog. 8: e1002373. Pubmed
  2. Ghosh, A., A. Choudhury, A. Das,N. S. Chatterjee, T. Das, R. Chowdhury, K. Panda, R. Banerjee and I. B. Chatterjee. 2012. Cigarette smoke induces p-benzoquinone-albumin adduct in blood serum: Implications on structure and ligand binding properties.Toxicology292:78-89 Pubmed
  3. Sabui, S., S. Dutta, A. Debnath, A. Ghosh, T. Hamabata, K. Rajendran, T. Ramamurthy, J.P. Nataro, D. Sur, M.M. Levine and N.S. Chatterjee. 2012. Real-time PCR-based mismatch amplification mutation assay for specific detection of CS6-expressing allelic variants of enterotoxigenic Escherichia coli and its application in assessing diarrheal cases and asymptomatic controls. J Clin Microbiol. 50: 1308-1312. Pubmed

2011

  1. Chatterjee, R., A. Ghosal, S. Sabui and N. S. Chatterjee. 2011. Three dimensional modelling of C-terminal loop of CssA subunit in CS6 of Enterotoxigenic Escherichia coli and its interaction with the 70 KDa domain of Fibronectin. Bioinformation 6: 307-310. Pubmed
  2. Wajima T., S. Sabui, M. Fukumoto, S. Kano, T. Ramamurthy, N. S. Chatterjee and T. Hamabata. 2011. Enterotoxigenic Escherichia coli CS6 gene products and their roles in CS6 structural protein assembly and cellular adherence. Microbial Pathogen. 51: 243-249. Pubmed

2010

  1. Sabui, S., A. Ghosal, S. Dutta, A.Ghosh, T. Ramamurthy, J.P. Nataro, T. Hamabata and N. S.Chatterjee. 2010. Allelic variation in colonization factor CS6 of enterotoxigenic Escherichia coli isolated from patients with acute diarrhoea and controls. J Med Microbiol. 59: 770-779. Pubmed

2009

  1. Bhowmick, D.C., B. Bal, N. S. Chatterjee, A. N. Ghosh and S. Pal. 2009. A low-GC Gram-positive Thermoanaerobacter-like bacterium isolated from an Indian hot spring contains Cr(VI) reduction activity both in the membrane and cytoplasm. J. Appl. Microbiol. 106: 2006-2016. Pubmed
  2. Ghosal, A., R. Bhowmick, R. Banerjee, S. Ganguly, S. Yamasaki, T. Ramamurthy, T. Hamabata and N. S. Chatterjee. 2009. Characterization and studies of the cellular interaction of native colonization factor CS6 purified from a clinical isolate of enterotoxigenic Escherichia coli. Infect. Immun. 77: 2125-2135. Pubmed

2008

  1. Bhowmick, R., A. Ghosal, B. Das, H. Koley, D. R. Saha, S. Ganguly , R. K. Nandy, R. K. Bhadra and N. S. Chatterjee. 2008. Intestinal adherence of Vibrio cholerae involves a coordinated interaction between colonization factor GbpA and mucin. Infect Immun. 76: 4968-4977. Pubmed

2007

  1. Ghosal, A., R. Bhowmick, R. K. Nandy, T. Ramamurthy and N. S. Chatterjee. 2007. PCR-based identification of common colonization factor antigens of enterotoxigenic Escherichia coli. J Clin Microbiol. 45: 3068-3071. Pubmed
  2. Bhowmick, R., A. Ghosal, and N. S. Chatterjee. 2007. Effect of environmental factors on expression and activity of chitinase genes of vibrios with special reference to Vibrio cholerae. J. Appl. Microbiol. 103: 97-108. Pubmed
  3. Sen, A., N. S. Chatterjee, M. A. Akbar, N. Nandi and P. Das. 2007. The 29-kilodalton thiol-dependent peroxidase of Entamoeba histolytica is a factor involved in pathogenesis and survival of the parasite during oxidative stress. Eukaryot. Cell. 6: 664-673. Pubmed

2004

  1. Akbar, M. A., N. S. Chatterjee, P. Sen, A. Debnath, A. Pal, T. Bera and P. Das. 2004. Genes induced by a high-oxygen environment in Entamoeba histolytica. Mol. Biochem. Parasitol. 133: 187-196. Pubmed

2003

  1. Ray, A., N. S. Chatterjee, S. K. Bhattacharya and T. Biswas. 2003. Porin of Shigella dysenteriae enhances mRNA levels for Toll-like receptor 2 and MyD88, up-regulates CD80 of murine macrophage, and induces the release of interleukin-12. FEMS Immunol. Med. Microbiol. 39: 213-219. Pubmed
  2. Saha, M.R., A. Palit, N. S. Chatterjee, P. Dutta, U. Mitra and S. K. Bhattacharya. 2003. A prospective study of phage types biotypes of Salmonella enterica serotype Typhi isolated from hospitalized children in Kolkata, India. Indian J. Med. Res. 117: 201-204. Pubmed
  3. Subramanian, V. S., N. S. Chatterjee and H. M. Said. 2003. Folate uptake in the human intestine: promoter activity and effect of folate deficiency. J. Cell. Physiol. 196: 403-408. Pubmed

2002

  1. Dey, S., V. S. Subramanian, N. S. Chatterjee, S. A. Rubin and H. M. Said. 2002. Characterization of the 5' regulatory region of the human sodium-dependent multivitamin transporter, hSMVT. Biochim. Biophys. Acta. 1574: 187-192. Pubmed

2001

  1. Chatterjee, N. S., S. A. Rubin and H. M. Said. 2001. Molecular characterization of the 5' regulatory region of rat sodium-dependent multivitamin transporter gene. Am. J. Physiol. Cell Physiol. 280: C548-C555. Pubmed

2000

  1. Said, H. M., N. S. Chatterjee, R. U. Haq, V. S. Subramanian, A. Ortiz, L. H. Matherly, F. M. Sirotnak, C. Halsted and S. A. Rubin. 2000. Adaptive regulation of intestinal folate uptake: effect of dietary folate deficiency. Am. J. Physiol. Cell Physiol. 279: C1889-1895. Pubmed

1999

  1. Chatterjee, N. S., C. K. Kumar, A. Ortiz, S. A. Rubin and H. M. Said. 1999. Molecular mechanism of the intestinal biotin transport process. Am. J. Physiol. Cell Physiol. 277: C605-613. Pubmed
  2. Said, H. M., A. Ortiz, C. K. Kumar, N. S. Chatterjee, P. K. Dudeja and S. Rubin. 1999. Transport of thiamine in human intestine: mechanism and regulation in intestinal epithelial cell model Caco-2. Am. J. Physiol. Cell Physiol. 277: C645-651. Pubmed

1998

  1. Chatterjee, M., N. S. Chatterjee, R. Datta, B. Datta and N. K. Gupta. 1998. Expression and activity of p67 are induced during heat shock. Biochem. Biophys. Res. Commun. 249: 113-117. Pubmed

1997

  1. Saha, D., S. Wu, A. Bose, N. S. Chatterjee, A. Chakraborty, M. Chatterjee and N. K. Gupta. 1997. Viral infection. II. Hemin induces overexpression of p67 as it partially prevents appearance of an active p67-deglycosylase in baculovirus-infected insect cells. Arch. Biochem. Biophys. 342: 373-382. Pubmed
  2. Gupta, S., A. Bose, N. S. Chatterjee, D. Saha, S. Wu, N. K. Gupta. 1997. p67 transcription regulates translation in serum-starved and mitogen-activated KRC-7 cells. J. Biol Chem. 272: 12699-12704. Pubmed
  3. Chatterjee, N. S., C. Zou, J. C. Osterman and N. K. Gupta. 1997. Cloning and characterization of the promoter region of a gene encoding a 67-kDa glycoprotein. J. Biol Chem. 272: 12692-12698. Pubmed

1995

  1. Gupta, S., S. Wu, N. S. Chatterjee, J. Ilan , J. Ilan, J. C. Osterman and N. K. Gupta. 1995. Regulation of an eukaryotic initiation factor-2 (eIF-2) associated 67 kDa glycoprotein (p67) and its requirement in protein synthesis. Gene Expr. 5: 113-122. Pubmed

1993

  1. Wu, S., S. Gupta, N. S. Chatterjee, R. E. Hileman, T. G. Kinzy, N. D. Denslow, W. C. Merrick, D. Chakrabarti, J. C. Osterman and N. K. Gupta. 1993. Cloning and characterization of complementary DNA encoding the eukaryotic initiation factor 2-associated 67-kDa protein (p67). J. Biol Chem. 268: 10796-10801. Pubmed

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