National Institute of Cholera and Enteric Diseases

Indian Council of Medical Research
Department of Health Research, Ministry of Health and Family Welfare, Government of India

NICED : Scientists

Dr. Moumita Dutta

Dr. Moumita Dutta

 

General Information
Name Dr. Moumita Dutta
Designation Scientist C
Date of joining ICMR 01 December 2017
Date of joining present post 01 December 2017
Discipline Electron Microscopy
Division  
Specialization  
Email : moumita.dutta@icmr.gov.in
Academic Qualification  
Graduation B.Sc in Chemistry (University of Calcutta)
Post Graduation M.Sc in Bio-chemistry (University of Calcutta)
Doctoral PhD in Life Sciences & Biotechnology: Characterization of Choleraphages using Electron Microscopy. ICMR-NICED, Kolkata.

Profile

Research Experience

B.Sc in Chemistry from University of Calcutta, M.Sc in Bio-chemistry from University of Calcutta and PhD in Life Sciences and Biotechnology from Jadavpur University. Specialization on electron microscopic techniques: negative stain analysis, immune-electron microscopy, ultramicrotomy, Kleinschmidt's protein monolayer technique, partial denaturation mapping of DNA, heteroduplex analysis of DNA, cryo-electron microscopy, single particle analysis with special emphasis on structural characterization of vibriophages. Postdoctoral research associate at Florida State University, USA to study HIV/SIV glycoprotein spikes and their complexes with broadly neutralizing antibodies using cryo-electron tomography and subtomogram averaging to provide structural information for designing better immunogen. Career development fellow at MRC-NIMR(London) later merged to Francis Crick Institute, London, UK to work on structural intermediates of bacterial replication initiation process and also retroviral capsid structures using cryo-electron tomography. Worked as senior staff scientist in cryo-electron microscopy at University of Sheffield, UK..
 

Research Interest

Research Interest: My research interest focused on solving structures of bacteriophages, enveloped viruses and associated membrane proteins to understand the structure-function relation and fundamental processes such as host-virus interaction and viral entry. I am also interested in structural analysis of protein and their complexes, immune-complexes to explain their functions in biological processes and related diseases. Surface structural changes and in-depth ulltrastructural changes caused by different enteric pathogens are also studied by scanning and transmission electron microscopy techniques. Primary research techniques used in our laboratory are negative staining analysis, Kleinschmidt’s protein monolayer technique of DNA, partial denaturation mapping of DNA, immunoelectron microscopy, ultramicrotomy for thin sectioning, cryo-electron microscopy and tomography, image processing and three-dimensional image reconstruction, scanning electron microscopy and atomic force microscopy. .

Membership/ Fellow of Professional Societies/ Associations

National

  1. Life member: Electron Microscope Society of India

International

  1. Member: Royal Microscopical Society
  2. Member: European Microscopy Society

Publications

2016

  1. Arbore, C., Dutta, M., Solscheid, C., Rosenthal, P., Molloy, J., Webb, M. Visualization of structural intermediates of replisome initiation complex formation on plasmid DNA replication (manuscript submitted).
  2. Mukherjee, CB., Dutta, M., Winkler, H., Liu, X., Roux, KH and Taylor, KA. Visualization of SIV envelope spike with automated particle picking on intact viruses by cryo-electron tomography (manuscript submitted).
  3. Ball NJ, Nicastro G, Dutta M, Pollard DJ, Goldstone DC, Sanz-Ramos M, Ramos A, Müllers E, Stirnnagel K, Stanke N, Lindemann D, Stoye JP, Taylor WR, Rosenthal PB, Taylor IA. Structure of a Spuma retrovirus Gag Central Domain Reveals an Ancient Retroviral Capsid. PLoS Pathog. 2016 Nov 9;12(11):e1005981.  Pubmed
  4. Gieras A, Linhart B, Roux KH, Dutta M, Khodoun M, Zafred D, Cabauatan CR, Lupinek C, Weber M, Focke-Tejkl M, Keller W, Finkelman FD, Valenta R. IgE epitope proximity determines immune complex shape and effector cell activation capacity. J Allergy Clin Immunol. 2016 May;137(5):1557-65 Pubmed

2014

  1. Dutta M, Liu J, Roux KH, Taylor KA. Visualization of retroviral envelope spikes in complex with the V3 loop antibody 447-52D on intact viruses by cryo-electron tomography. J Virol. 2014 Nov;88(21):12265-75.  Pubmed

2012

  1. Rathinakumar R, Dutta M, Zhu P, Johnson WE, Roux KH. Binding of anti-membrane-proximal gp41 monoclonal antibodies to CD4-liganded and –unliganded human immunodeficiency virus type 1 and simian immunodeficiency virus virions. J Virol. 2012 Feb;86(3):1820-31.   Pubmed

2007

  1. Dutta M, Ghosh AN. Physicochemical characterization of El Tor Vibriophage S20. Intervirology. 2007;50(4):264-72. Pubmed

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